This category of products presents professional models of laboratory microscopes, medical microscopes and biological microscopes equipped with a special dark field condenser. All these models can be successfully applied in native blood microscopy and in hemoscanning, LBA, for studying microobjects in medicine, biology, and veterinary medicine.
The task of a dark-field condenser is to collect a stream of light into a beam and direct it from the side to the viewed micro-object (smear of living blood). As a result, a blood smear looks completely different than when viewed in a bright field.
In a dark field, transparent objects (blood plasma) are black or dark in color, and opaque objects (various blood cells, bacterial flora, parasitic forms, other microinclusions in plasma) receive brightly glowing edges - reflecting light incident from the side. The side lighting effect makes it easy to identify the bacterial flora, parasitoforms, other microinclusions in the plasma and evaluate the shape and functional disorders of various blood cells. Translucent objects (white blood cells, i.e. lymphocytes, white blood cells, granulocytes) are also clearly visible and contrasting. The image in the dark field also gets an interesting three-dimensional effect, as in 3D.
To obtain an image in a dark field, it is necessary to apply immersion oil to the dark field condenser, bring the condenser to the bottom of the glass until the oil and the glass come in contact, a smear with a cover glass has already been placed on the glass. Immersion oil is also applied to the coverslip, into which the 100X oil lens is immersed. Only with such a scheme for using a dark-field condenser can we obtain the largest possible image of micro-objects in a dark field, sufficient to identify micro-objects. The process of ensuring the desired thickness of the layer of immersion oil both from the bottom and from the top of the smear is complex and time-consuming.
The use of a dark-field condenser makes the microscope more expensive and requires a specialist to set up the microscope to display the correct dark-field image of the smear on the screen or in the eyepieces.
Also, if you plan to use a digital camera to output a high-definition dark-field image onto a monitor, photo and video documentation in a dark field, then cameras with increased photosensitivity are required, which significantly increases the cost of the camera and, therefore, the entire set of equipment. The camera control and the entire process of visualization and photo video documentation should be entrusted to the Dianel-Micro program.
Thus, only experienced researchers can use dark-field microscopes and examine micro-objects (blood, saliva, urine, lymph) in a dark field. By purchasing a dark-field microscope, you assume the corresponding responsibility. Since the 2000s, in Russia, native blood microscopy and hemoscanning have been performed in a bright field, because it’s easier, cheaper and no less obvious.
Advantages of a dark-field condenser microscope:
An interesting three-dimensional image of cells with the 3D effect, emphasizing the change in the shape of the cells and convenient for a specialist;
Bright luminous contours of cells;
Good visibility of translucent objects in the plasma;
Good visibility of small inclusions in the plasma (bacteria, larvae, other parasitic forms);
Disadvantages of a microscope with a dark field condenser:
A fairly expensive component of the microscope;
The complexity of installation under a microscope, and regular installation and dismantling is required - often, alternating use of a light field condenser and a dark field condenser are required, which change places;
The difficulty of setting a dark field, which leads to an increase in the time of testing the patient;
Image unusual for the inexperienced eye of the patient - black blood cells, luminous edges, dark background;
Printing images of a dark field leads to increased consumption of a cartridge of a printing device;
There is many years of successful practice of using a bright field capacitor for the purpose of a qualitative blood analysis, assessing the functional state of blood cells, hemoscanning, and native blood microscopy, and therefore, the demand and popularity of a dark field condenser in Russia has significantly decreased. At this time, in Western countries, specialists in integrative medicine, holistic medicine, complementary medicine and nutritionists actively use a dark-field condenser as part of expensive Olympus, Nikon, Carl Zeiss microscopes, etc. for a qualitative analysis of the functional state of the blood.